The Chemopreventive Effects of Saussurea salicifolia through Induction of Apoptosis and Phase II Detoxification Enzyme(Pharmacognosy)

スポンサーリンク

概要

• 論文の詳細を見る

• The ethanol extract of the aerial part of the Mongolian medicinal plant Saussurea salicifolia induced a dose-dependent cell growth inhibition in both human gastric adenocarcinoma AGS cells and mouse hepatoma Hepa 1c1c7 cells (IC_<50>=30.22 and 116.96μg/ml), respectively. The extract induced an apoptosis in AGS cells inference from the externalization of the phosphatidylserine, the increase of the sub G0/G1 content (%) and the apoptotic morphological changes including membrane blebbing, the formation of apoptotic bodies and chromatin condensation. In order to identify active substances causing the apoptosis, we further isolated major compounds present in Saussurea salicifolia and 7 compounds were isolated including a sesquiterpene lactone, cynaropicrin, 3 lignans (trachelogenin, matairesinol and arctigenin) and 3 lignan glycosides (tracheloside, matairesinoside and arctiin). In general the lignan aglycones were more cytotoxic than their lignan glycosides in both AGS cells and Hepa 1c1c7 cells. Cynaropicrin not only showed the most potent cytotoxicity among the 7 major compounds but also it induced an apoptosis and a weak G2/M arrest in AGS cells. Arctigenin had the second-best cytotoxicity among 7 major compounds, and induced an apoptosis. In order to evaluate the induction of the phase II detoxification enzyme, we measured the induction of quinone reductase activity of the extract, fractions and compounds in Hepa 1c1c7 cells. The ethyl acetate fraction and arctigenin showed the strongest cancer chemopreventive activity (chemoprevention index=9.88 and 7.57, respectively). These data suggest that the extract as well as the lignan compounds (especially arctigenin) originated from Saussurea salicifolia may be served as potential cancer chemopreventive agents for prevention or treatment of human cancers.

• 2007-12-01

著者

• Lee Hee

  Natural Products Res. Center Korea Inst. Of Sci. And Technol. Gangneung Inst.

• Lee Hee

  Natural Products Research Center Korea Institute Of Science And Technology

• Kim Chul

  Natural Products Res. Center Korea Inst. Of Sci. And Technol. Gangneung Inst.

• Nho Chu

  Natural Products Research Center, Gangneung Institute, Korea Institute of Science and Technology

• Lee Hee

  Natural Products Res. Center Gangneung Inst. Korea Inst. Of Sci. And Technol.

• Nho Chu

  Natural Products Res. Center Gangneung Inst. Korea Inst. Of Sci. And Technol.

• KANG Kyungsu

  Natural Products Research Center, Korea Institute of Science and Technology

• LEE Saet

  Natural Products Research Center, Korea Institute of Science and Technology

• TUNSAG Jigjidsuren

  Institute of Chemistry and Chemical Technology

• BATSUREN Dulamjav

  Institute of Chemistry and Chemical Technology

• Kang Kyungsu

  Natural Products Research Center Korea Institute Of Science And Technology

• Lee Saet

  Natural Products Res. Center Gangneung Inst. Korea Inst. Of Sci. And Technol.

• Kim Chul

  Natural Products Res. Center Gangneung Inst. Korea Inst. Of Sci. And Technol.

関連論文

• The Rapid Identification of Isoflavonoids from Belamcanda chinensis by LC-NMR and LC-MS
• Aldose Reductase and Advanced Glycation Endproducts Inhibitory Effect of Phyllostachys nigra(Pharmacognosy)
• Bi-Functional Induction of the Quinone Reductase and Cytochrome P450 1A1 by Youngiasides via Nrf2-ARE and AhR-XRE Pathways
• The Chemopreventive Effect of Taxifolin Is Exerted through ARE-Dependent Gene Regulation(Molecular and Cell Biology)
• The Chemopreventive Effects of Saussurea salicifolia through Induction of Apoptosis and Phase II Detoxification Enzyme(Pharmacognosy)
• Anti-Inflammatory Effects of Gomisin N, Gomisin J, and Schisandrin C Isolated from the Fruit of Schisandra chinensis

スポンサーリンク