Characterization of Neurons Differentiated from Mouse Embryonic Stem Cells Using Conditioned Medium of Dorsal Root Ganglia(CELL AND TISSUE ENGINEERING)

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概要

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• Mouse embryonic stem (ES) cells have the pluripotent ability to differentiate in vitro into various cell lineages, including neurons. Adding chick dorsal root ganglion (DRG) conditioned medium (CM) to the culture medium promotes the differentiation of ES cells into neurons. We determined the types of neurons that differentiate from ES cells. The addition of DRG-CM caused nearly half of all ES cells on the periphery of the colony sphere to differentiate into neurons. Immunofluorescence analysis showed that the neurons that differentiated from ES cells were mainly motor, GABAergic, serotonergic, and cholinergic neurons. Of particular note, flow cytometry showed that approximately 50% of βIII-tubulin-positive neurons were motor neurons. This indicates that DRG-CM induces ES cells to differentiate into motor neurons as target of DRG neurons (sensory neurons).

• 公益社団法人日本生物工学会の論文
• 2007-10-25

著者

• Kitazawa Ayako

  Graduate School Of Life Sciences Toyo Univ.

• Kitazawa Ayako

  Graduate School Of Life Sciences Toyo University

• Shimizu Norio

  Graduate School Of Life Sciences Toyo University

• Kitazawa Ayako

  Graduate School of Life Sciences, Toyo University

関連論文

• Neurite Outgrowths of Neurons with Neurotrophin-Coated Carbon Nanotubes(CELL AND TISSUE ENGINEERING)
• Neurite Outgrowths of Neurons Using Neurotrophin-Coated Nanoscale Magnetic Beads(MEDICAL BIOTECHNOLOGY)
• Differentiation of Mouse Embryonic Stem Cells into Neurons Using Conditioned Medium of Dorsal Root Ganglia(MEDICAL BIOTECHNOLOGY, Advances in Biomedical Science and Engineering)
• Characterization of Neurons Differentiated from Mouse Embryonic Stem Cells Using Conditioned Medium of Dorsal Root Ganglia(CELL AND TISSUE ENGINEERING)

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