Differentiation of Mouse Embryonic Stem Cells into Neurons Using Conditioned Medium of Dorsal Root Ganglia(MEDICAL BIOTECHNOLOGY, <Special Issue>Advances in Biomedical Science and Engineering)

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概要

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• Mouse embryonic stem (ES) cells, which are continuously growing cell lines, have a pluripotent ability to differentiate into various cell lineages in vitro including neurons. We investigated the effects of chick dorsal root ganglion (DRG) conditioned medium (CM) and nerve growth factor (NGF) on the directed differentiation of ES cells into neurons. Because DRGs from 8-day-old chick embryos are often used in bioassays of neurotrophic factors, DRGs may release soluble factors that can induce ES cell differentiation into neurons in a culture broth. When cultivated in a Dulbecco's modified Eagle's medium (DMEM)/F-12K medium containing DRG-CM or NGF, the ES cell colonies clearly showed neurite outgrowths. Of particular significance, the immunofluorescence analysis of ES cell colonies using an anti-βIII-tubulin antibody indicated that the addition of DRG-CM effectively promoted the differentiation of ES cells into neurons. We confirmed the effect of DRG-CM addition on ES cell differentiation into neurons via neuronal stem cells by the immunofluorescence analysis of ES cell colonies. Thus, DRG-CM appeared to effectively promote ES cell differentiation into neurons.

• 公益社団法人日本生物工学会の論文
• 2005-07-25

著者

• Shimizu Norio

  東洋大学 生命科学部

• Kitazawa Ayako

  Graduate School Of Life Sciences Toyo Univ.

• SHIMIZU NORIO

  Graduate School of Life Sciences, Toyo University

• Shimizu Norio

  Graduate School Of Life Sciences Toyo University

関連論文

• Neurite Outgrowths of Neurons with Neurotrophin-Coated Carbon Nanotubes(CELL AND TISSUE ENGINEERING)
• Neurite Outgrowths of Neurons Using Neurotrophin-Coated Nanoscale Magnetic Beads(MEDICAL BIOTECHNOLOGY)
• Differentiation of Mouse Embryonic Stem Cells into Neurons Using Conditioned Medium of Dorsal Root Ganglia(MEDICAL BIOTECHNOLOGY, Advances in Biomedical Science and Engineering)
• Characterization of Neurons Differentiated from Mouse Embryonic Stem Cells Using Conditioned Medium of Dorsal Root Ganglia(CELL AND TISSUE ENGINEERING)

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