Quantitative analysis of 15p+ in a human diploid cell strain, TIG-7, by laser scanning cytometry
スポンサーリンク
概要
- 論文の詳細を見る
A molecular cytogenetic study of the human diploid cell strain TIG-7 was performed by laser scanning cytometry combined with fluorescence in situ hybridization (FISH). A heteromorphic chromosome 15 (15p+) carrying a huge ribosomal RNA gene (rDNA) cluster was found with a DNA content of about 1.45 times that of the normal homologue. This result was comparable to our previous finding by bivariate flow karyotyping that the G+C content of 15p+ was higher than that of chromosome 15 in this cell strain. The possible advantages of laser scanning cytometry for chromosomal DNA analysis were discussed in comparison with flow cytometry.
- 財団法人染色体学会の論文
著者
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SATOH Takatomo
Chromosome Research Center,Olympus Optical Co.Ltd
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ISHIDATE Motoi
Chromosome Research Center,Olympus Optical Co.Ltd
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Yamamoto Kiyotaka
Department of Cell Biology, Tokyo Metropolitan Institute of Gerontology
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Yamamoto Kiyotaka
Dept. Cell Biol. Tokyo Metropolitan Inst. Gerontl.
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Yamamoto Kiyotaka
Department Of Cell Biology Tokyo Metropolitan Institute Of Gerontology
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Satoh Takatomo
Chromosome Res. Cent. Olympus Optical Co. Ltd.
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Ishidate M
Olympus Optical Co. Ltd. Tokyo Jpn
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Ishidate Motoi
Chromosome Research Center Olympus Optical Co.ltd
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Miura Kunihiko
Chromosome Res. Cent. Olympus Optical Co. Ltd.
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ISHIDATE Motoi
Chromosome Research Center (CRC), OLYMPUS Optical Co., Ltd.
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