Direct Determination of Oxidation State of Gold Deposits in Metal-Reducing Bacterium Shewanella algae Using X-Ray Absorption Near-Edge Structure Spectroscopy (XANES)(ENVIRONMENTAL BIOTECHOLOGY)

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概要

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• X-ray absorption near-edge structure spectroscopy (XANES) was successfully employed to determine the gold valence in the metal-reducing bacterium Shewanella algae after exposure to a 1mM aqueous HAuCl_4 solution for 10-120min. XANES spectra revealed the oxidation state of gold in the bacterial cells to be Au(0) without any contribution from Au(III), demonstrating that S. algae cells can reduce AuCl_4^- ions to elemental gold. Transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analysis confirmed that gold nanoparticles 5-15 nm in size were deposited in the periplasmic space of the bacterial cells; a preferable, cell surface location for the easy recovery of biogenic nanoparticles.

• 2007-06-25

著者

• URUGA Tomoya

  Japan Synchrotron Radiation Research Institute

• Takahashi Yoshio

  Department Of Cardiology Mie University Graduate School Of Medicine

• NAGAMINE Shinsuke

  Department of Chemical Engineering, Kyoto University, Katsura Campus

• KONISHI Yasuhiro

  Department of Chemical Engineering, The School of Engineering, Osaka Prefecture University

• Nomura Toshiyuki

  Department Of Chemical Engineering Osaka Prefecture University

• Konishi Yasuhiro

  Osaka Prefecture Univ. Osaka Jpn

• Konishi Yasuhiro

  Department Of Chemical Engineering Osaka Prefecture University

• Konishi Yasuhiro

  Dept. Of Chem. Eng. Osaka Pref. Univ.

• Nomura Toshiyuki

  Osaka Prefecture Univ. Osaka Jpn

• Tsukiyama Takeshi

  Department of Chemical Engineering, Osaka Prefecture University

• Saitoh Norizoh

  Department of Chemical Engineering, Osaka Prefecture University

• Saitoh Norizoh

  Department Of Chemical Engineering Osaka Prefecture University

• Tsukiyama Takeshi

  Department Of Chemical Engineering Osaka Prefecture University

• Nagamine Shinsuke

  Department Of Chemical Engineering Osaka Prefecture University

• Nagamine Shinsuke

  Department Of Chemical Engineering Faculty Of Engineering Kyoto University

• Takahashi Yoshio

  Department Of Agricultural Chemistry The University Of Tokyo

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