Alpha-Ketoglutarate Stimulates Procollagen Production in Cultured Human Dermal Fibroblasts, and Decreases UVB-Induced Wrinkle Formation Following Topical Application on the Dorsal Skin of Hairless Mice(Molecular and Cell Biology)

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概要

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• Alpha-ketoglutarate is a key intermediate in the Krebs cycle, and a rate-limiting cofactor of prolyl-4-hydroxylase. It also has a potent effect on increasing the proline pool during collagen production, but the details underlying the boosting effect on collagen production by α-ketoglutarate remain as yet unreported. To investigate the effects of a-ketoglutarate on procollagen production and wrinkle formation, we conducted experiments in cultured human dermal fibroblasts and UVB-irradiated hairless mice. Based on ELISA measurements, α-ketoglutarate (10 μM) stimulated procollagen production in fibroblasts by 25.6±4.6% compared to vehicle (dH_2O)-treated control cells. Also, we demonstrated that α-ketoglutarate increased activities of prolidase, which is known to play an important role in collagen metabolism, in fibroblasts and N-benzyloxycarbonyl-_L-proline (Cbz-Pro), prolidase inhibitor, inhibited procollagen synthesis by α-ketoglutarate in fibroblasts. To determine the effect of topically applied a-ketoglutarate on wrinkle formation, α-ketoglutarate (1%) and vehicle (70% propylene glycol, 30% ethanol) were applied on the dorsal skin of UVB-induced hairless mice for twelve weeks. We found that α-ketoglutarate decreased wrinkle formation upon long-term topical application. These results suggest that α-ketoglutarate diminishes UVB-induced wrinkle formation by increasing collagen production, through a pathway that involves prolidase activation. Therefore, application of a-ketoglutarate may represent an effective anti-wrinkle agent for the cosmetic field.

• 公益社団法人日本薬学会の論文
• 2007-08-01

著者

• Hwang Jae

  韓国

• Hwang Jae

  Skin Research Institute Amorepacific R&d Center

• HWANG Jae

  Amorepacific Corporation R&D Center

• Chang Ih

  Amorepacific Corporation R&d Center

• SON Eui

  AmorePacific Corp/R&D Center

• KIM Hyaekyoung

  AmorePacific Corp/R&D Center

• CHOI Gyu

  Korea Food & Drug Administration

• LEE Byoungseok

  AmorePacific Corporation R&D Center

• Son Eui

  Amorepacific Corp/r&d Center

• Choi Gyu

  Korea Food & Drug Administration

• Kim Hyaekyoung

  Amorepacific Corp/r&d Center

• Lee Byoungseok

  Amorepacific Corporation R&d Center

• Hwang Jae

  Skin Research Institute Amorepacific Corporation/r&d Center

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• Alpha-Ketoglutarate Stimulates Procollagen Production in Cultured Human Dermal Fibroblasts, and Decreases UVB-Induced Wrinkle Formation Following Topical Application on the Dorsal Skin of Hairless Mice(Molecular and Cell Biology)

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