Purification and Properties of Phospholipase D from Streptomyces lydicus(Biological Chemistry)
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概要
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Phospholipase D was purified from Streptomyces lydicus by treatment with CM-Toyopearl 650M, column chromatography on DEAE-Toyopearl 650M and Toyopearl HW50F, and by chromatofocusing. The enzyme preparation was electrophoretically homogeneous and the molecular weight of the enzyme was estimated to be 56,000. Its isoelectric point was around pH 7.4. The enzyme was most active at pH 6.0 and at around 55℃. It was stable between pH 4 and 7, and below 60℃.
- 社団法人日本農芸化学会の論文
- 1990-05-23
著者
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Yano H
Tohoku Univ. Sendai Jpn
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Muramoto Y
Ntt Network Innovation Laboratories Ntt Corporation
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Miyamoto Yutaka
Research Group For Analytical Sciences Nuclear Science And Engineering Directorate Jaea
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SHIMBO Kikuo
Research Laboratory of Honen Corporation
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YANO Hideki
Research Laboratory of Honen Corporation
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