Expression and Stability of a β-Glucosidase Gene of Ruminococcus albus in Zymomonas mobilis(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A β-glucosidase gene of Ruminococcus albus was introduced into Zymomonas mobilis NRRL B-14023, ATCC29191 and B69, on a newly constructed vector, a derivative of pZA22, by conjugal transfer with the helper plasmid pRK2013. Z. mobilis strains carrying this gene showed similar levels of intracellular β-glucosidase activity (per culture volume) to Escherichia coli carrying the same gene on pBR322. The stability of a hybrid plasmid carrying the β-glucosidase gene in Z. mobilis was examined through serial transfer to antibiotic-free medium; 91〜93% after the first transfer and 60〜85% after the fourth transfer of the Z. mobilis cells were β-glucosidase-producing ones in which the hybrid plasmid was retained.
- 社団法人日本農芸化学会の論文
- 1989-03-23
著者
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Misawa Norihiko
Central Laboratories Of Key Technology Kirin Brewery Co. Ltd.
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Misawa Norihiko
Central Laboratories For Frontier Technol. Kirin Holdings Co. Ltd.
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Nakamura Katsumi
Central Laboratories Of Key Technology Kirin Brewery Co. Ltd.
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Nakamura Katsumi
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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