Enzymatic Phosphorylation of Soybean Proteins by Protein Kinase(Food & Nutrition)
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概要
- 論文の詳細を見る
Soybean proteins were subjected to phosphorylation with cyclic adenosine monophosphate-dependent protein kinase (A-kinase). As a result, acidic subunits of the 11S fraction were found to be phosphorylated by A-kinase. To estimate the effect of the phosphorylation, 11S acidic subunits were isolated and subjected to A-kinase phosphorylation. The optimal enzyme amount and Mg^<2+> concentration for the phosphorylation of 11S acidic subunits were determined to be 1.5U/ml and 1.6mM, respectively. The rate of phosphorylation was 2mol/mol acidic subunits (MW 38,000) under the above conditions. The protein structures of 11S acidic subunits, as determined from UV and CD spectra, were slightly affected by the enzymatic phosphorylation.
- 社団法人日本農芸化学会の論文
- 1989-12-23
著者
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MOTOKI Masao
Central Research Laboratories, Ajinomoto Co., Inc.
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Motoki Masao
Central Research Laboratories Ajinomoto Co. Inc.
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SEGURO Katsuya
Central Research Laboratories, Ajinomoto Co., Inc.
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Seguro Katsuya
Central Research Laboratories Ajinomoto Co. Inc.
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