Toxins of a Wild Candida Killer Yeast with a Novel Killer Property(Microbiology & Fermentation Industry)

概要

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The killer toxic substance of Candida SW-55 was separated into two components, I and II, by CM-Sepharose CL-6B column chromatgraphy. They were purified 20 700-fold and 11 100-fold from the culture filtrate of SW-55, respectively. Each purified toxin gave a marked glycoprotein band with molecular mass of 36kDa on SDS/polyacrylamide gel electrophoresis. Toxins I and II had almost the same isoelectric points, 3.4〜3.7 and 3.3〜3.8, respectively. Toxin I had strong killer activity against Saccharomtces cerevisiae, Candida glabrata, Hansenula anomala, and Rhodotorula rubra (MIC 0.2〜0.3μg/ml), and moderate activity against Kluyveromtces lactis (MIC 2.5μg/ml) and pichia membranaefaciens (MIC 0.6μg/ml) but bacteria, fungi, and the other yeasts tested were not affected by toxin I even at the high concentration of 20 μg/ml. Toxin II turned out to be less active than toxin I and the MIC for S. cerevisiae Epernay was 0.4〜0.5μg/ml.
社団法人日本農芸化学会の論文
1988-11-23