Structures of Integrated DNA Containing Human Adenovirus E1A Gene in Transgenic Mice(Biological Chemistry)

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概要

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• One of the DNAs, Charon10-gE1A-2, pSV-gpt-gE1A, or Ad12 E1A, was microinjected separately into pronuclei of fertilized mouse eggs and fourteen transgenic mouse lines were obtained. The structures of integrated DNAs in these transgenic mice were analyzed by Southern blot hybridization. Head-to-head and tail-to-tail structures were found, although the injected molecules had a tendency to join each other in head-to-tail tandem forms when multiple copies were integrated. The analyses of the mice containing pSV2-gpt-gE1A showed that most of the restriction sites at the end of the injected molecules were restored at the tandem junctions. Furthermore, the integrated DNAs at the junctions to the host chromosomal DNA were deleted and/or rearranged.

• 社団法人日本農芸化学会の論文
• 1988-10-23

著者

• Ninomiya Takashi

  Research Institute Of Life Science Snou Brand Milk Products Co. Ltd.

• Hoshi Masaki

  Research Institute Of Life Science Snou Brand Milk Products Co. Ltd.

• YUKI Atsushi

  Research Institute of Life Science, Snou Brand Milk Products, Co., Ltd.

• Yuki A

  Snow Brand Milk Products Co. Ltd. Tochigi Jpn

• Yuki Atsushi

  Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.

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• Transgenic mice overexpressing soluble osteoclast differentiation factor (sODF) exhibit severe osteoporosis
• Structures of Integrated DNA Containing Human Adenovirus E1A Gene in Transgenic Mice(Biological Chemistry)
• Application of the Polymerase Chain Reaction (PCR) and Inverted PCR to Analyze Transgenes in Transgenic Mice(Biological Chemistry)
• Direct Sequencing of Flanking Regions of a Transgene Amplified by Inverted PCR(Biological Chemistry)
• Instability of Inverted Repeats in a Transgenic Mouse Line during Germline Transmission

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