Instability of Inverted Repeats in a Transgenic Mouse Line during Germline Transmission

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概要

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• In addition to head-to-tail tandem repeats of injected pSV2-gpt-gE1A DNA, head-to-head and tail-to-tail tandem structures were detected in a transgenic mouse, pEl A/3, which showed the existence of inverted repeats. The injected DNA molecules in the founder mouse were segregated into two groups in its progeny. One group had about 80 copies of pSV2-gpt-gE1A and the other had 20 copies per diploid genome. Both the high and the low copy number lines contained inverted repeats. Breeding experiments showed that the inverted repeats in about half of the transgenic progeny were lost during the transmission from generation to generation. Progeny of the mice that lost the inverted repeats carried only head-to-tail tandem repeats. Besides the inverted sequences, another portion of the transgene in the high copy line was found to be lost during germline transmission but independently.

• 社団法人 日本農芸化学会の論文

著者

• Ninomiya Takashi

  Research Institute Of Life Science Snou Brand Milk Products Co. Ltd.

• Hoshi Masaki

  Research Institute Of Life Science Snou Brand Milk Products Co. Ltd.

• Yuki Atsushi

  Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.

関連論文

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• Structures of Integrated DNA Containing Human Adenovirus E1A Gene in Transgenic Mice(Biological Chemistry)
• Application of the Polymerase Chain Reaction (PCR) and Inverted PCR to Analyze Transgenes in Transgenic Mice(Biological Chemistry)
• Direct Sequencing of Flanking Regions of a Transgene Amplified by Inverted PCR(Biological Chemistry)
• Instability of Inverted Repeats in a Transgenic Mouse Line during Germline Transmission

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