Purification and Properties of Wall-bound α-Glucosidase from Suspension-cultured Rice Cells(Biological Chemistry)

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概要

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• Wall-bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured rice cells with Sumyzyme C and Pectolyase Y-23 and isolated by a procedure including fractionation with ammonium sulfate, Sephadex G-100 column chromatography, CM-cellulose column chromatography, Sephadex G-200 column chromatography, and preparative disc gel electrophoresis. The molecular weight of the enzyme was 64,000. The enzyme readily hydrolyzed maltose, maltotriose, and amylose, but hydrolyzed isomaltose and soluble starch more slowly. The Michaelis constant for maltose of the enzyme was estimated to be 0.272mM. The enzyme produced panose as the main α-glucosyltransferred product from maltose.

• 社団法人日本農芸化学会の論文
• 1987-12-23

著者

• Konno Haruyoshi

  Institute For Agricultural And Biological Sciences Okayama University

• Yamasaki Y

  Okayama Univ. Kurashiki Jpn

• Yamasaki Yoshiki

  Institute For Agricultural And Biological Sciences Okayama University

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• Two Forms of α-Glucosidase from Soybean Callus
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