Sweet Potato Starch Phosphorylase-Purification and Characterization(Biological Chemistry)

概要

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Starach phosphorylase was purified from either freshly harvested or stored roots of sweet potato (Ipomoea batatas (L.) Lam. cv Tainon 65). Both enzyme preparations in their native state showed on polyacrylamide gel electrophoresis a cluster of about six closely located activity bands, which had common antigenic determinants as they were simultaneously probed by monoclonal antibodies. The molecules of enzymes from stored roots were smaller than those from fresh roots. However, the two enzyme preparations had completely fused precipitin lines in double diffusion assays with an antiserum raised against the fresh root preparation. One large subunit and several small ones were found for both enzyme preparations. The small subunits appeared to be the degradation products of the large ones as revealed by peptide mapping and immunoblotting. Immunofluorescence microscopy showed that the enzyme was present in the amyloplasts and cell walls of root storage parenchyma.
社団法人日本農芸化学会の論文
1987-01-23