Solubility Properties of a 65-kDa Peptide Prepared by Restricted Digestion of Myosin with Astacin-like Squid Metalloprotease(SHORT COMMUNICATION,Biochemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Substructure of the myosin rod and its correlation to filament formation is largely based on studies of proteolytic digests and expressed proteins. However, tryptic digestion of myosin always produces polymorphous peptides. Consequently, it is difficult to determine the relation between myosin substructure and filament formation. Similarly, filament formation with recombinant myosin protein is also difficult to interpret because it is never clear whether the recombinant protein folds like the native protein. We recently reported a novel metal protease isolated from squid liver, astacin-like squid metalloprotease (ALSM), which can specifically hydrolyze in vitro myosin heavy chain. In the present study, we examined the solubility properties of the 65-kDa peptide and light meromyosin (LMM) prepared by ALSM isoform II and trypsin digestion, respectively. The 65-kDa peptide is much less soluble than LMM under physiological conditions, even though the length of 65-kDa peptide is shorter than that of LMM. These results suggest that a novel substructure of myosin drives filament assembly.
- 社団法人日本動物学会の論文
- 2004-02-25
著者
-
Kanzawa Nobuyuki
Department Of Chemistry Faculty Of Science And Technology Sophia University
-
Tsuchiya Takahide
Department Of Chemistry Faculty Of Science And Technology Sophia University
-
Yabuta Hiroko
Department of Chemistry, Faculty of Science and Technology, Sophia University
-
Fujimi Takahiko
Department of Chemistry, Faculty of Science and Technology, Sophia University
-
Yabuta Hiroko
Department Of Chemistry Faculty Of Science And Technology Sophia University
-
Fujimi Takahiko
Department Of Chemistry Faculty Of Science And Technology Sophia University
-
KANZAWA Nobuyuki
Department of Biology, Faculty of Science, Chiba University
関連論文
- Phase I and II pharmacokinetic and pharmacodynamic study of the proteasome inhibitor bortezomib in Japanese patients with relapsed or refractory multiple myeloma
- High molecular weight lectin isolated from the mucus of the giant African snail Achatina fulica
- High Molecular Weight Lectin Isolated from the Mucus of the Giant African Snail Achatina fulica
- Changes in Light Scattering Intensity of Fish Holo-, Apo-and Reconstituted Myoglobins under Thermal Denaturation
- Effect of Heating on Autoxidation Rate of Fish Holo-and Reconstituted Myoglobins
- Studies on Thermal Denaturation Profiles of Holoand Reconstituted Myoglobins from Bonito and Sperm Whale
- Studies on Thermal Denaturation of Fish Apomyoglobins using Differential Scanning Calorimetry, Circular Dichroism, and Fluorescence
- IDENTIFICATION OF ASTACIN-LIKE SQUID METALLOPROTEASE IN CUTTLEFISH, SEPIA ESCVLENTA(Taxonomy and Systematics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- The Gelsolin/Fragmin Family Protein Identified in the Higher Plant Mimosa pudica
- Purification and Characterization of a Novel Isoform of Myosinase from Spear Squid Liver
- Distribution of Myosinase I and Myosinase II in Tissues of Coleoidea
- Clinical Effects of Infusing Anti-Epstein -Barr Virus (EBV)-Specific Cytotoxic T-Lymphocytes into Patients with Severe Chronic Active EBV Infection
- The Generation of Immunocompetent Dendritic Cells From CD34^+ Acute Myeloid or Lymphoid Leukemia Cells
- Cloning and Biochemical Characterization of Astacin-Like Squid Metalloprotease
- Effects of additives on the thermal gelation of Japanese common squid natural actomyosin
- Studies on Thermal Denaturation of Fish Myoglobins using Differential Scanning Calorimetry, Circular Dichroism, and Tryptophan Fluorescence
- Change in the Actin Cytoskeleton during Seismonastic Movement of Mimosa pudica
- High Molecular Weight Lectin Isolated from the Mucus of the Giant African Snail Achatina fulica
- Antiserum against the Synthetic Polypeptide Fragment of Dystrophin Cross-reacts with Myofibrillar C-protein
- Inhibitory effect of niboshi on food intake
- Isolation and Characterization of Sea Sponge Myosin
- Effect of paramyosin on invertebrate natural actomyosin gel formation
- Biochemical characterization of Mimosa villin
- Solubility Properties of a 65-kDa Peptide Prepared by Restricted Digestion of Myosin with Astacin-like Squid Metalloprotease(SHORT COMMUNICATION,Biochemistry)
- Characterization of Myosin Isolated from Bodywall Smooth Muscle of the Annelid, Urechis unicinctus.
- Cloning and Biochemical Characterization of Astacin-Like Squid Metalloprotease.