Cloning of a New S-RNase and Development of a PCR-RFLP System for the Determination of the S-genotypes of Japanese Pear

概要

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Japanese pear exhibits gametophytic self-incompatibility that is controlled by the multiallelic S-locus. The S-locus encodes polymorphic S-RNase for the pistil-part S determinant in Japanese pear. Information about the S-genotypes is important for the selection of pollen donors for fruit production and breeding of Japanese pear cultivars. We determined the S-genotypes of three Japanese pear cultivars, 'Kinchaku' (S^4S^k), 'Natsuhikari' (S^3S^4) and 'Wakahikari' (S^3S^4), and an F_1 hybrid cultivar between a Japanese pear cultivar and a European pear cultivar, 'Oharabeni' (S^9S^e), by PCR-RFLP analysis of S-RNase. A new S^k-RNase allele was identified in 'Kinchaku', and full length cDNA encoding S^k-RNase was sequenced. The S^k-RNase was not effectively amplified by the previously published primers. We designed a new PCR primer combination for the amplification of the S^k-RNase allele in addition to the S^1- to S^9-RNase alleles of Japanese pear, and developed a PCR-RFLP system for identifying the S^1- to S^9- and S^k-alleles.
日本育種学会の論文