Molecular Mapping of Non-Brittle Rachis Genes <I>btr1</I> and <I>btr2</I> using STS Markers in Barley
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概要
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Brittle rachis is an important trait to study the domestication process in barley. Ancient farmers preferred to have non-brittle barley for cultivation, and domesticated barley lost the brittle rachis after a process of human selection. In this study, partial linkage maps in the region of non-brittle rachis genes <I>btr1 </I>and <I>btr2 </I>were constructed using two wild × cultivar F<SUB>2</SUB> populations. STS markers derived from AFLP markers <I>e09m25-08</I>, <I>e30m07-09 </I>and <I>e50m21-01</I>, which showed no recombination with the <I>btr1 </I>and <I>btr2 </I>loci in recombinant inbred lines (RILs), showed recombination with the <I>btr1 </I>and <I>btr2 </I>loci in the two F<SUB>2</SUB> populations. Though there were few differences in map distances, orders of the markers were highly conserved. OUH602 (wild) × Kanto Nakate Gold (cultivar) was suitable for highresolution mapping of the <I>btr1 </I>locus due to unambiguous phenotypes in segregating F<SUB>2</SUB> plants.
- 独立行政法人 国際農林水産業研究センターの論文
著者
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Komatsuda Takao
Plant Genome Res. Unit National Inst. Of Agrobiological Sciences (nias)
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Koba Takato
Faculty Of Horticulture Chiba University
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VIDYA SARASWATHI
Plant Genome Research Unit, National Institute of Agrobiological Sciences (NIAS)
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AZHAGUVEL Perumal
Plant Genome Research Unit, National Institute of Agrobiological Sciences (NIAS)
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SENTHIL Natesan
Plant Genome Research Unit, National Institute of Agrobiological Sciences (NIAS)
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