LC/MSによるプロリダーゼ欠損症患者の尿中イミノジペプチドの分析

概要

論文の詳細を見る
Liquid chromatography-mass spectrometry with atmospheric pressure ionization interface (LC/API-MS) could sensitively detect various synthetic iminodipeptides, distinguish-ing X-Pro from Pro-X on their retention times in mass chromatograms and mass spectra. Iminodipeptides (X-Pro) in the urine of patients with prolidase deficiency were successfully quantified with calibration curves of synthetic iminodipeptides which were so linear. LC/API-MS has also revealed the fact that iminodipeptides containing a C-terminal hydroxyproline (Gly -Hyp, Pro-Hyp) are excreted in the urine of normal human as well as in that of patients with prolidase deficiency, and the patients excrete much more Gly-Hyp and Pro-Hyp than normal human, Not to mention the patients, the excretion of X-Hyp in normal human urine might result from the very low prolidase activity against X-Hyp in comparison with that against X-Pro.
高知大学の論文
1991-12-10