A Rapid Determination Method for Anthocyanin Profiling in Grape Genetic Resources
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概要
- 論文の詳細を見る
A rapid determination method has been developed to elucidate the anthocyanin profiles in grape genetic resources. It consists of crude extraction of skin anthocyanins with 50% aqueous acetic acid and analysis of reversed-phase HPLC with a visible spectrum detector. A total of 21 anthocyanins could be rapidly identified without the need for multi-step extraction and equipment such as diode array spectroscopy and/or mass spectrometry. Analysis of 17 colored cultivars exhibited diversified anthocyanin profiles in the levels of hydroxylation, methylation, glycosidation, and acylation of aglycones, suggesting that significant inter- and intraspecific variations exist in grape genetic resources. The anthocyanin profiles for a grape cultivar from the same location appeared to be quite similar between vines or years.
- 園芸学会の論文
著者
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MITANI Nobuhito
Department of Citrus Research, National Institute of Fruit Tree Science
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Yamada Masahiko
Department Of Internal Medicine Anjo Kosei Hospital
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Mitani Nobuhito
Department Of Grape And Persimmon Research National Institute Of Fruit Tree Science
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Shiraishi Mikio
Department Of Grape And Persimmon Research National Institute Of Fruit Tree Science:(present Address
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Ueno Toshihito
Department of Grape and Persimmon Research National Institute of Fruit Tree Science
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Yamada Masahiko
Department Of Cardiology Ogaki Municipal Hospital
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Yamada Masahiko
Department Of Grape And Persimmon Research National Institute Of Fruit Tree Science
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MITANI Nobuhito
Department of Applied Biological Chemistry, The University of Tokyo
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Yamada Masahiko
Department of Applied Chemistry and Molecular Science, Iwate University
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