Expression of a Single-chain Antibody against Indole-3-acetic Acid in Escherichia coli
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概要
- 論文の詳細を見る
A hybridoma cell line that produces a monoclonal antibody specific for indole-3-acetic acid (IAA) was prepared. The DNA fragments coding the variable regions of the light and the heavy chains of the antibody were prepared by PCR using the cDNA of the antibody as a template. A chimera DNA for a single chain variable fragment (scFv) was constructed, and expressed in Escherichia coli. The scFv antibody expressed in E. coli as well as the original monoclonal antibody showed a specific binding to IAA.
- 社団法人 日本農芸化学会の論文
- 2004-07-23
著者
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Matsumoto Ryoji
Department Of Physics Graduate School Of Science Chiba University
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Matsumoto Ryoji
Department Of Citrus Research National Institute Of Fruit Tree Science
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MITANI Nobuhito
Department of Citrus Research, National Institute of Fruit Tree Science
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Mitani N
Department Of Citrus Research National Institute Of Fruit Tree Science
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MITANI Nobuhito
Department of Applied Biological Chemistry, The University of Tokyo
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