Modification of Limonoid Metabolism in Suspension Cell Culture of Citrus

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概要

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• For the metabolic engineering of bio-functional substances in Citrus, metabolic changes of limonoids were investigated in embryogenic cell cultures of Citrus and Fortunella. When embryogenic cells of Citrus and Fortunella were cultured for four weeks in the liquid subculture medium containing nomilin, most species converted nomilin to obacunone and limonin. In addition, calamin group limonoids were produced in Fortunella and Calamondin cells. Deacetylnomilin was produced from nomilin in the cells of C. ichangensis and its related species. Exogenously added limonin into medium was not metabolized. The embryoid formation in C.aurantium did not affect the limonoid metabolism. All cells cultured without the presence of nomilin or limonin did not generate any limonoids. Furthermore, transgenic C. sinensis cell harboring a chimeric limonoid UDP-glucosyltransferase (limonoid GTase) gene was generated. The conversion of limonin into limonin 17-β-D-glucopyranoside (LG) demonstrated an efficient assay system for the transgene.

• 日本植物細胞分子生物学会の論文
• 2002-12-03

著者

• Endo Tomoko

  Department of Pediatrics, School of Medicine, Gunma University

• Matsumoto Ryoji

  Department Of Physics Graduate School Of Science Chiba University

• Matsumoto Ryoji

  Department Of Citrus Research National Institute Of Fruit Tree Science

• Matsumoto Ryoji

  Department Of Citrus Research National Institute Of Fruit Tree Science National Agricultural Researc

• KITA Masayuki

  Department of Citriculture, National Institute of Fruit Tree Science

• OMURA Mitsuo

  Department of Citriculture, National Institute of Fruit Tree Science

• MORIGUCHI Takaya

  Department of Citriculture, National Institute of Fruit Tree Science

• Endo-inagaki Tomoko

  Department Of Citrus Research National Institute Of Fruit Tree Science National Agricultural Researc

• Endo Tomoko

  Department Of Material Science Faculty Of Science Himeji Institute Of Technology

• Omura M

  Okitsu Branch Fruit Tree Research Station Ministry Of Agriculture Forestry And Fisheries

• Omura M

  Department Of Biological Sciences Faculty Of Agriculture Shizuoka University

• SHIMADA Takehiko

  Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Resea

• HIDAKA Tetsushi

  Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Resea

• HASEGAWA Shin

  Western Regional Research Center, Agricultural Research Service, U. S. Department of Agriculture

• Omura Mitsuo

  Department Of Biological Sciences Faculty Of Agriculture Shizuoka University

• Shimizu Tokurou

  Department Of Citrus Research National Institute Of Fruit Tree Science National Agricultural Researc

• Shimada Takehiko

  Department Of Citrus Research National Institute Of Fruit Tree Science National Agricultural Researc

• Moriguchi Takaya

  Department Of Citrus Research National Institute Of Fruit Tree Science National Agricultural Researc

• Kita Masayuki

  Department Of Citrus Research National Institute Of Fruit Tree Science National Agricultural Researc

• Omura Mitsuo

  Faculty Of Agriculture Shizuoka University

• Shimizu Tokurou

  Department Of Citrus Research National Institute Of Fruit Tree Science

• Hidaka Tetsushi

  Department Of Citrus Research National Institute Of Fruit Tree Science National Agricultural Researc

• Endo Tomoko

  Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization

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