Accumulation of Mercury in Transgenic Tobacco Expressing Bacterial Polyphosphate(Analytical Biochemistry)

概要

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The feasibility of transgenic tobacco, engineered to express bacterial polyphosphate (polyP), for phytoreme-diation of mercury pollution was evaluated. T_3 progeny of the transgenic tobacco produced a large amount of polyP in leaves and showed a relatively high resistant phenotype to Hg^<2+> than its wild-type progenitors. These results suggest that the integrated ppk gene, encoding polyphosphate kinase (PPK), a key enzyme for polyP biosynthesis, is stably conserved in tobacco genome, and translated to active PPK which catalyzed biosynthesis of polyP, and suggest that polyP is capable of reducing the cytotoxicity of Hg^<2+>, probably via chelation formation with polyP. The transgenic tobacco expressing polyP accumulated significantly more mercury than its wild-type progenitors from Hg^<2+>-containing agar medium and simulated soils without taxing the tobacco plants suggesting that the transported Hg^<2+> was accumulated as a less toxic Hg-polyP complex in the tobacco tissues. Based on the results obtained in the present study, the polyP-mediated accumulation of mercury from mercurial-contaminated soils may provide an ecologically compatible approach for phytoremediation of mercury pollution.
公益社団法人日本薬学会の論文
2006-12-01