Development of a luminescence-based biosensor for detection of methylmercury
スポンサーリンク
概要
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A recombinant whole-cell bacterial sensor for highly selective and sensitive detection of the bioavailable methylmercury in the environment was constructed. The biosensor carries luciferase gene, luxAB, from Vibrio harveyi as a reporter under the control of the mercury inducible regulatory part of mer-operon from Pseudomonas K-62 plasmid pMR26. In addition, a merB gene encoding organomercurial lyase which cleaves the C-Hg bond of methylmercury to give Hg2+ was coexpressed in the sensor. The resulting bacterial sensor responded specifically to methylmercury, and the lowest detectable concentration of methylmercury was 10 pM with 1 ml sample in the optimized assay conditions. This detection limit is enough to detect this compound in many contaminated and some pristine environmental samples.
- 2010-04-01
著者
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PAN-HOU Hidemitsu
Faculty of Pharmaceutical Sciences, Setsunan University
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NAGATA Takeshi
Faculty of Pharmaceutical Sciences, Setsunan University
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MURAOKA Toshiaki
Faculty of Pharmaceutical Sciences, Setsunan University
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KIYONO Masako
School of Pharmaceutical Sciences, Kitasato University
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Kiyono Masako
School Of Pharmaceutical Sciences Kitasato University
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Kiyono M
Faculty Of Pharmaceutical Sciences Setsunan University
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Muraoka Toshiaki
Faculty Of Pharmaceutical Sciences Setsunan University
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Pan-hou Hidemitsu
Faculty Of Pharmaceutical Sciences Setsunan University
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Pan-hou Hidemitsu
Facul.of Pharm.sci. Setsunan Univ.
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Nagata Takeshi
Faculty Of Pharmaceutical Sciences Setsunan University
関連論文
- DNA Sequence and Expression of a Defective mer Operon from Pseudomonas K-62 Plasmid pMR26
- Development of a luminescence-based biosensor for detection of methylmercury
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- Formation of Adenosine 3'-Monophosphate in Rat Liver Mitochondria
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- Genetic Engineering of Bacteria for Environmental Remediation of Mercury
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- Genetic Engineering of Transgenic Tobacco for Enhanced Uptake and Bioaccumulation of Mercury
- Role of MerC, MerE, MerF, MerT, and/or MerP in resistance to mercurials and the transport of mercurials in Escherichia coli
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