ミュータンス連鎖球菌の非水溶性多糖合成酵素遺伝子のDNA塩基配列とアミノ酸配列

概要

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Mutans streptococci produce water-insoluble-glucan synthetase (glucosyltransfer-ase- I ), which is a pathogenic factor causing dental caries. The glucosyltransferase-I gene was isolated from Streptococcs sobrirtus ATCC33478^T and analyzed. The analysis showed that the gene existed in the Pst I fragment (6838 bp) of the S. sobrinus chromosome. The data of the DNA sequence showed that one open reading frame (DNA position, 1033-5805) coded 1590 amino acids, ribosome binding site (RBS, AGGAGGA) and promoter sequences (TTGTAA for -35,TAAAAT for -10) were located prior to the coding region, and two termination sequences were found after TAA stop codon. The amino-acid sequence indicated that the enzyme molecular size was 176,049,thirty-eight amino-acid residues from N terminus was a signal peptide for the enzyme secretion, and DSIRVDAVD (amino-acid position, 442-450) was a binding site of glucose-residue of sucrose. In the C-terminal one third of the sequence 71 glysine residues constructed nine G-repeat units. That C-terminal region is known as a glucan-binding region.
鹿児島大学の論文
1996-03-25