Crucial Role of SDF-1/CXCR4 Interaction in the Recruitment of Transplanted Dermal Multipotent Cells to Sublethally Irradiated Bone Marrow
スポンサーリンク
概要
- 論文の詳細を見る
Our previous study indicated that dermal multipotent cells (DMCs) could engraft into bone morrow (BM) of rats with sublethal irradiation and promote hematopoietic recovery after being transplanted systemically, but the mechanisms determining the recruitment of DMCs to the irradiation injured BM remain unclear. In the present study, we investigated the role of stromal cellderived factor-1 (SDF-1)/CXCR4 interaction in this process. Male DMCs were isolated and transplanted into female rats systemically, and by employing quantitative real-time TaqMan polymerase chain reaction for the sex-determining region of Y chromosome, it was found that the amount of DMCs in BM of rats with sublethal irradiation was about 3 times more than that of normal rats (P<0.01). Incubation of DMCs with AMD3100 before transplantation, which specifically blocks binding of SDF-1 to its endogenous receptor CXCR4, diminished recruitment of DMCs to the injured BM by 57.2±5.5% (P<0.05). In addition, it was confirmed that the expression of SDF-1 in injured BM was up-regulated when compared with that in normal BM, and in vitro analysis revealed that BM extracts from irradiated rats had a strong chemotactic effect on DMCs, which decreased significantly when DMCs were pre-incubated with AMD3100 (P<0.05). These data suggest that transplanted DMCs were recruited more frequently to irradiation-injured BM than normal BM and the interactions of SDF-1/CXCR4 played an important role in this process.
- 日本放射線影響学会の論文
著者
-
LI Nan
Institute of combined injury, State Key Laboratory of Trauma, Burns and combined injury, School of M
-
SU Yong-Ping
Institute of combined injury, State Key Laboratory of Trauma, Burns and combined injury, School of M
-
CHENG Tian-Min
Institute of combined injury, State Key Laboratory of Trauma, Burns and combined injury, School of M
-
Zong Zhao-wen
State Key Laboratory Of Trauma Burns And Combined Injury Institute Of Combined Injury Of Pla College
-
Zong Zhao-wen
Institute Of Combined Injury School Of Military Preventive Medicine State Key Laboratory Of Trauma B
-
Ran Xin-ze
State Key Laboratory Of Trauma Burns And Combined Injury Institute Of Combined Injury Of Pla College
-
Ran Xin-ze
Institute Of Combined Injury School Of Military Preventive Medicine State Key Laboratory Of Trauma B
-
Ran Xin
Institute Of Combine Injury School Of Preventive Medicine State Key Laboratory Of Trauma Burns And C
-
Su Yong-ping
State Key Laboratory Of Trauma Burns And Combined Injury Institute Of Combined Injury Of Pla College
-
Su Yong-ping
Institute Of Combined Injury School Of Military Preventive Medicine State Key Laboratory Of Trauma B
-
Su Yong
Institute Of Combine Injury School Of Preventive Medicine State Key Laboratory Of Trauma Burns And C
-
Cheng Tian-min
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury School Of Mili
-
Cheng Tian-min
Institute Of Combine Injury School Of Preventive Medicine Third Military Medical University
-
AI Guo-Ping
Institute of combined injury, State Key Laboratory of Trauma, Burns and combined injury, School of M
-
XU Hui
Institute of combined injury, School of Military Preventive Medicine, State Key Laboratory of Trauma
-
Li Nan
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury School Of Mili
-
Li Nan
Institute Of Combined Injury School Of Military Preventive Medicine State Key Laboratory Of Trauma B
-
Xu Hui
Institute Of Combined Injury School Of Military Preventive Medicine State Key Laboratory Of Trauma B
-
Xu Hui
中華人民共和国
-
Ai Guo-ping
Institute Of Combined Injury State Key Laboratory Of Trauma Burns And Combined Injury School Of Mili
-
Ai Guo-ping
Institute Of Combined Injury School Of Military Preventive Medicine State Key Laboratory Of Trauma B
-
Xu Hui
Institute Of Analytical Science Department Of Chemistry Nanjing University
関連論文
- Effect of hBD2 Genetically Modified Dermal Multipotent Stem Cells on Repair of Infected Irradiated Wounds
- Protective Effect of Atorvastatin on Radiation-induced Vascular Endothelial Cell Injury In Vitro
- Transplantation of Dermal Multipotent Cells Promotes the Hematopoietic Recovery in Sublethally Irradiated Rats
- CXCR4 Gene Transfer Enhances The Distribution of Dermal Multipotent Stem Cells to Bone Marrow in Sublethally Irradiated Rats
- Crucial Role of SDF-1/CXCR4 Interaction in the Recruitment of Transplanted Dermal Multipotent Cells to Sublethally Irradiated Bone Marrow
- STM Nano-Lithography with SiO_2 Mask
- SL-1-1 The intestinal injuries by radiation, and the related work in China(Radiation Biology Research in Asia, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Effects of the Nerve Growth Factor on the Survival and Wound Healing in Mice with Combined Radiation and Wound Injury
- Estimation of the Genetic Risks of Exposure to Ionizing Radiation in Humans : Current Status and Emerging Perspectives
- Enantiomeric Separation of Basic Drugs with Partially Filled Serum Albumin as Chiral Selector in Capillary Electrophoresis
- Mitigating nitrous oxide emissions from a maize-cropping black soil in northeast China by a combination of reducing chemical N fertilizer application and applying manure in autumn(Fertilizers and soil amendments)