Identification of parental chromosomes and changes of artificial, intergeneric F_1 hybrid between Dendranthema horaimontana and Nipponanthemum nipponicum by fluorescence genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH)

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概要

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• The maternal Dendranthema horaimontana (2n=18) and the paternal Nipponanthemum nipponicum (2n=18) were artificially crossed and produced F_1 hybrid plants of which the strains' embryo rescued and axenic cultured in vitro and later acclimatized in vivo. Genomic in situ hybridization (GISH) distinguished respective genome for the two parents in the chromosome complement of the F_1 hybrid plants and showed the nine largest chromosomes were related to N. nipponicum while the nine smallest chromosomes were related to D. horaimontana. Some of the hybrid plants showed a non-reciprocal translocation in certain somatic chromosomes of N. nipponicum, which could occur during the process of somatic cell division after the fertilization without any progress of the meiosis. Simultaneous bicolor of GISH and fluorescence in situ hybridization (FISH) using the digoxigenin-labelled total genomic DNA of N. nipponicum and biotin-labeled probes of pTa71 of 45S rDNA and 5S rDNA distinguished the chromosomes of NOR and 5S rDNA in the genome of respective species in the chromosome complement of the F_1 hybrid. One FISH signal of 45S and that of 5S rDNA were hybridized in separated sites with each probe in two of the nine chromosomes of D. horaimontana, while the FISH signals of 45S and 5S rDNA were hybridized and co-localized at the 45S rDNA site in one of the nine chromosomes of N. nipponicum in the chromosome complement of their F_1 hybrid. The FISH signal of the 5S rDNA showed instability between existence and disappearance in the chromosomes of N. nipponicum in the F_1 hybrid plants. GISH snowed that the chromosomes of D. horaimotana and N. nipponicum were in spatial separation in chromosomal domains during the intherphase and mitotic cell division.

• 財団法人染色体学会の論文

著者

• KONDO Katsuhiko

  Department of Electrical Engineering, Faculty of Engineering, Osaka University

• Magdy Hussein

  Laboratory Of Plant Chromosome And Gene Stock Graduate School Of Science Hiroshima University:depart

• Kondo Katsuhiko

  Department Of Botany Faculty Of Science El-minia University

関連論文

• Measurement of Surface Fermi Level in Phosphidized GaAs
• Identification of parental chromosomes and changes of artificial, intergeneric F_1 hybrid between Dendranthema horaimontana and Nipponanthemum nipponicum by fluorescence genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH)
• Physical mapping of 45S rDNA loci by fluorescent in situ hybridization and evolution among polyploid Dendranthema species
• Identification of mutation and homologous chromosomes in four cultivars of Dendranthema grandiflora by physical mapping of 5S and 45S rDNAs using fluorescence in situ hybridization

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