Studies on the Antibiotics from Actinomycetes. An Antibiotic Pigment from Streptomyces F-23b

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A strain F-23b, which resembles Streptomyces flavoelus, has been found to produce an antibiotic yellow pigment. This pigment was isolated in crystalline form' and identified as a pigment of actinomycin groups. The pigment can be produced in the glucose or starch bouillon medium and Czapek-Dox medium added peptone. And the pigment was isolated from the broth following the method of benzene extraction or acid clay adsorption. Crude pigment was purified by alumina chromatography. The pigment is bright red crystals, and it was melted at 244-245℃ (decomp.). Water solution of the pigment have λmax. at 237-238 mμ and 438 mμ. It was easily inactivated by sunlight. Empirical formula of the pigment was C_<49>H_<71>N_9O_<15>. Biological properties of the pigment are coinsided with actinomycin pigment. Hydrolysis of the pigment gave amino acids and carbonyl compounds. Amino acids were serine, sarcosine, N-methyl valine, proline, tyrosine, valine and iso-leucine, and they were identified by paper chromatography. Non-peptide fraction (carbonyl compound) gave despeptide actinomycin C as acetyl derivative and red crystals, showed m.p. 204-205℃ (decomp.). and λmax. at 232 mμ and 255 mμ in benzene solution, and its 2,4-dinitro-phenyl hydrazine derivative showed m.p. 111-113℃.
大阪府立大学の論文
1961-12-20