Studies on Vitamin B_6 : Part, I. The colorimetric determination of pyridoxine

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概要

• 論文の詳細を見る

• (1) The determination of pyridoxine was attempted with 2, 6-dichloro-quinone chloroimide (imide) reagent in a phase of water-isopropyl alcohol. (2) Better results were obtained for the color development of pyridoxine indophenol by using pH 7.8-veronal buffer in 20 parts and isopropyl alcohol in 70 parts to the total volume. (3) The time for maximum color intensity and the stability of intensity was affected by the reaction temperature. The time was 6 minutes at 30℃ and 14 minutes at 15℃. The stability was better in a lower temperature. (4) The procedure of determination was as follows: 1ml of pyridoxine solution, 5ml of isopropyl alcohol, 2ml of imide reagent and 2ml of pH 7.8 veronal buffer were mixed at 20℃. After 10 minutes, the color intensity was measured at 650mμ by means of a spectrophotometer. (5) Pyridoxine solution of 2-20μg per ml can be determined with ±1.0 average deviation and with the sensitivity as same as that of HOCHBERGS'S method and three times that of SCUDI'S method.

• 大阪府立大学の論文
• 1959-12-31

著者

• Kawabata Akiko

  Baika Women's Junior College.

• TAKEUCHI Yosiichi

  Laboratory of Nutrition Chemistry, College of Agriculture

• IWASA Yosaburo

  Laboratory of Nutrition Chemistry, College of Agriculture

• KAWABATA Akiko

  Laboratory of Nutrition Chemistry, College of Agiculture

• Iwasa Yosaburo

  Laboratory Of Nutrition Chemistry College Of Agriculture

• Takeuchi Yosiichi

  Laboratory Of Nutrition Chemistry College Of Agriculture

関連論文

• Studies on Vitamin B_6 : An Electrophoretic Study on Pyridoxine Phosphate
• Studies on Vitamin B_6 : Part, I. The colorimetric determination of pyridoxine
• The Lipid Metabolism in Castor beans (1) The activity of thiokinase in germinating castor beans

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