直流・脈流によるゾーリムシの細胞賦活に関する実験的研究

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From a viewpoint to research on the longevity of an organism, the authors considered an organism to be a thermodynamical closed system. As far as we have this viewpoint, the longevity of an organism has relation to the second law of the thermodynamics on entropy increase and when this law is operated reversely, it is possible for the longevity of an organism to be unlimited. Now suppose that a protein developed into a unicellular and the latter has completed a systematic evolution, the beginning of our study is Paramecium caudatum. This study follows the preceding report by Imura and Washizuka, "contraction phenomenon on protein by direct current (puls. current) (1965, 1966)". Paramecium caudatum which we used as the material in our experiments has a connection with "the problem on the endless living theory of unicellular" by Woodruff (1911), Calkins (1913), and Sonneborn (1954). Therefore Paramecium caudatum is very interesting to us. The experimental studies on Paramecium caudatum were done as follows. I. In the pre-experiments 1. The test method by Inewara (rice plant hay) medium 2. The fission and the longevity of Paramecium caudatum at different temperatures II. In the main experiments Cell vitality phenomenon of Paramecium caudatum by direct current and pulsating current 1. Test method of slide glass (SG method) 2. Test method of hall slide glass (HS method) The results of pre-experiments (1) Paramecium caudatum were cultivated in Inewara infussion. Water (water 50cc/1g Inewara) was boiled for 30 minutes and after this several Inenwaras were added to the Inewara infussion. The opening of the test tube was covered with a piece of paraphine paper. This medium was flourished with food bacteria, Bacillus subtilus. We used this medium in our experiments two days after it had been made. (2) At higher temperatures than 20℃, the fission of Paramecium caudatum was active. On the contrary, at lower temperatures than 20℃, the fission rate is low. The shorter the population longevity becomes, the lower the temperature is and the longer the former becomes, the higher the latter is. The experimental results of the main experiments are as follows. (3) In this experiment the difference between cell vitality phenomenon of Paramecium caudatum by pulsating current and that of Paramecium caudatum by direct current, was seen and also the phenomenon that alternating current treatment obstructs this species was made clear (Table 5-10). When the fission rate of control is 1, that rate of Paramecium caudatum by pulsating current treatment is 1.3 (mean) in SG method, and is 1.1 (mean) in HS method. On the other hand, by direct current treatment, it is 0.8 (mean) in SG method and is 1.0 (mean) in HS method. The fission rate of Paramecium caudatum by alternating current is 0.8 (mean) in SG method and is 0.9 (mean) in HS method. The results of these experiments are shown in Fig.3 and Fig.8 and they are summarized as follows. When the fission rate of control is l and it is shown as 45°line, most of the points by pulsating current treatment are above the line (about 80%). The points by direct current treatment are near the 45°line, and also the points by alternating current treatment are under the 45°line (about 68%). Judging from this phenomenon, alternating current treatment seems to work as an adverse effect to Paramecium caudatum. Through above experiments, cell vitality phenomenon by pulsating current was made clear. So we want to research on these problems in more systematically evolved organisms.
大分県立芸術文化短期大学の論文
1966-12-10

直流・脈流によるゾーリムシの細胞賦活に関する実験的研究

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