Effects of Aphidicolin on Entamoeba histolytica Growth and DNA Synthesis

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概要

• 論文の詳細を見る

• We have detected and characterized DNA polymerase activity in cell extracts from trophozoites of Entamoeba histolytica and have found that the activity of E. histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1: IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [^3H] thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.

• 東海大学の論文
• 1998-08-29

著者

• Kobayashi Seiki

  Department of Parasitology, School of Medicine, Keio University

• Takeuchi Tsutomu

  Department of Parasitology, School of Medicine, Keio University

• Kobayashi Seiki

  Department Of Parasitology School Of Medicine Keio University

• Takeuchi T

  Department Of Tropical Medicine And Parasitology School Of Medicine Keio University

• MAKIOKA Asao

  Department of Tropical Medicine, Jikei University School of Medicine

• OHTOMO Hiroshi

  Department of Tropical Medicine, Jikei University School of Medicine

• Ohtomo Hiroshi

  Department Of Tropical Medicine Jikei University School Of Medicine

• Takeuchi Tsutomu

  Keio Univ. School Of Medicine Tokyo Jpn

• Makioka A

  Jikei Univ. School Of Medicine Tokyo Jpn

• Makioka Asao

  Department Of Tropical Medicine Jikei University School Of Medicine

• Ohtomo H

  Department Of Tropical Medicine Jikei University School Of Medicine

• Takeuchi Tsutomu

  Department Of Obstetrics And Gynecology Tokyo Rinkai Hospital

• Kobayashi Seiki

  Keio Univ. School Of Medicine Tokyo Jpn

• Takeuchi Tsutomu

  Department Of Astronomy Faculty Of Science Kyoto University

• OHTOMO Hiroshi

  Department of Medical Zoology, Faculty of Medicine, Tokyo Medical and Dental University

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