陰イオン吸着樹脂による臨床材料中のPCR阻害物質の除去と結核菌直接検出への適用

概要

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The inhibitory effect on PCR was remarkably showed by the presence of clinical materials such as pleural effusion, blood and sputum. So, to eliminate inhibitors and to directly detect Mycobacterium tuberculosis DNA, an anion binding resin (GeneReleaser) was tested. In case of pleural effusion or blood, PCR performance was recovered especially with GeneReleaser treatment. But the inhibition by sputum was so hard that PCR-product can be recognized after 8-fold dilution. For directly detection of M. tuberculosis DNA, five sputum from tuberculosis patients were treated by NALC-NaOH washing procedure and followed by GeneReleaser treatment. The authors could obtain the reasonable results. The treatment of GeneReleaser was very rapid, simple and clean more than that of phenol-chloroform in popular.
神戸大学の論文