マイクロウェーブ照射による染色法の改良 : 3.グロコット染色

概要

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Grocott's methenamine silver method is a well-known method to stain fungi. This method takes long, about 3 hours, to complete the process. In this study, the authors applied microwave irradiation (MWI) (500W) to speed it up. Among the staining processes, 2 steps were taken by MWI. First of all, the oxidation by chromic acid with slide inside was heated up to 60℃ by MWI. In this step, the heat absorption water (300ml) at MWI was effective to prevent the specimen from detaching from the slide glass, although MWI time was prolonged. Secondly, the methenamine silver solution with slide inside was heated up to 60°C by MWI. After MWI, the solution was left at room temperature for 2 minutes. In this step, the temperature distribution affected the quality of the stain. To avoid it, the authors frequently changed the position of the container at MWI or stirred the solution gently after MWI. By this method, the quality of the stain was compatible with the conventional method and the time needed was shortened within 10 minutes.
神戸大学の論文