マイクロウェーブ照射による染色法の改良 : 1.グリメリウス染色

概要

論文の詳細を見る
Since Grimelius reported the staining method in 1868, this Grimelius method has been widely applied to stain the neuroendocrine granules. The problem is that this method takes long and the stain is uneven. In this study, the authors used microwave irradiation (MWI) (500W) to shorten time and avoid the uneven stain in the pancreatic islets. Among the staining processes, 2 steps were taken by MWI. First of all, The immersion step in the silver solution was shorten to 10 minutes at room temperature after it was heated up to 70 °C with slide inside by MWI. Secondly, the reductive solution was heated up to 45℃ with the slide inside by MWI. After it reached 45°C, it was gently stirred and left at room temperture for 2 minutes. By this method, the uneven stain was not recognized. By repeating these 2 steps succeedingly 3 times, the argyrophilic neuroendocrine granules in the pancreatic islets were well stained without uneven stain. This MWI was effective to shorten the time and to avoid uneven stain.
神戸大学の論文