マイクロウェーブ照射による染色法の改良 : 2.クリューバー・バレラ染色

概要

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Kluver-Barrera's method is a well-known and widely used method to stain the Schwann's sheath of the central nervous system. This method takes long hours, over night, to complete the process. Therefore, the authors used microwave irradiation (MWI) (500W) to shorten time. Since luxol-fast blue (LEB) is dissolved in the 95% alcohol, the MWI time is critical to heat the solution up to the set temperature. MWI with heat absorption water beside the LFB solution can heat it slowly. In this study, MWI was applied for 50 seconds with 300ml heat absorption water beside the solution and it was heated up to approximately 60℃. After MWI, the slide was left in the same LFB solution for more than 5 minutes at room temperature to make the stain tight and to prevent the slide from drying when it was taken out from the solution for the next step, by this MWI application, the Kluver-Barrera's method could be completed within one hour, compared with the conventional method that takes over night.