EFFECTS OF NH_3/NH^+_4 AT THE LEVEL OF HELICOBACTER PYLORI-POSITIVE GASTRIC CONTENTS ON GASTRODUODENAL MUCOSAL PERMEABILITY

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Background: Cytotoxicity of NH_3/NH^+_4, at the concentration detected in Helicobacter pylori-infected stomach has not been yet well investigated in vivo. Aim: We sought to determine whether luminal administrations of NH_3/NH^+_4 change gastric or duodenal mucosal permeability. Methods: Male Sprague-Dawley rats were fasted and anesthetized. NH_4Cl (15-30mM), or NH_3 (0.5-30 mM) solution was perfused into the rat gastric or duodenal lumen. NH_3/NH^+_4 was also tested for aggravating effect on acid-induced injury. During luminal perfusion with NH_3/NH^+_4, 0.3 N and 0.05 N HCl were perfused into gastric and duodenal lumen to induce mucosal injuries, respectively. As an index of epithelial damage, the blood-to-lumen clearance of chromium 51-labeled ethylenediaminetetraacetic acid (^<51>Cr-EDTA) was monitored continuously. Results: NH_4 Cl did not increase gastroduodenal mucosal permeability. NH_3 (10-30 mM) significantly increased both gastric and duodenal clearance of ^<51>Cr-EDTA in a dosedependent manner. NH_3 solution at the concentrations which did not cause any mucosal injury significantly aggravated both gastric and duodenal injury caused by HCl. NH_4 Cl (pH=6.9-7.0), which did not change permeability of gastric mucosa, significantly aggravate gastric damage induced by acid perfusion, but not duodenal damage. Conclusions: These findings suggest that; 1) Hp-producing NH_3/NH^+_4 at the concentration detected in Hp-infected stomach may be directly and/or indirectly (through the acid injury) damaging both the gastric and duodenal mucosa, and that 2) NH^+_4 may be more caustic to the gastric mucosa than to duodenal mucosa.
名古屋市立大学の論文
2005-03-28

EFFECTS OF NH_3/NH^+_4 AT THE LEVEL OF HELICOBACTER PYLORI-POSITIVE GASTRIC CONTENTS ON GASTRODUODENAL MUCOSAL PERMEABILITY

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