THE STUDY OF A POLYPEPTIDE ENCODED FROM A PUTATIVE OPEN READING FRAME IN THE NEGATIVE STRAND OF GB VIRUS-C/HEPATITS G VIRUS

概要

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The core protein of GBvirus-C/Hepatitis G virus (GBV-C/HGV) remains to be identified. Comparisons of nucleotide sequences of available GBV-C/HGV genes have revealed only one possible common new open reading frame (new-orf) which is longer than 300 nucleotides found in negative strand sequences. In order to characterize the polypeptide by new-orf, we prepared a fused protein of the GBV-C/HGV-new-orf polypeptide by attaching a 6 xHistidine-tag at the C terminal. The GBV-C/HGV-new-orf protein was detected in cytoplasm using an anti-His-tag antibody. However the GBV-C/HGV-new-orf protein was not detected by 7 GBV-C/HGV-positive sera, including serum of patient from which the GBV-C/HGV-new-orf cDNA was prepared.
名古屋市立大学の論文
2000-03-31