A Gene Encoding a Putative Acyl-transferase Involved in Pungency of Capsicum
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概要
- 論文の詳細を見る
Pungency in the fruit of pepper (Capsicum spp.) is due to the accumulation of capsaicinoids. We isolated two genes characterized as putative Capsicum acyl-transferase genes (Catf-1 and Catf-2) from the placenta of pungent pepper, which displayed consensus motifs of acyl-transferase, HD and DFGG. The expression pattern of Catf-1 coincided with the accumulation of capsaicinoids in pungent pepper. The Catf-1 was expressed in placenta of pungent pepper fruit, but not in other organs, such as leaf, flower, seed and pericarp. Accumulation of mRNA of Catf-1 varied with the developmental stages of the pungent pepper fruits. Genomic PCR and Southern blot studies revealed the presence of a sequence deletion at the 5' end of the Catf-1 coding region in the non-pungent line. In the F_2 population obtained from a cross between a non-pungent strain ('mGTY-1') and a pungent strain ('277 long'), the genotype at the Catf-1 locus cosegregated with the phenotype for fruit pungency. These results suggest that Catf-1 is a candidate gene differentiating pungent from non-pungent peppers.
- 日本育種学会の論文
著者
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Sasakuma Tetsuo
Kihara Institute For Biological Research And Graduate School Of Integrated Science Yokohama City Uni
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Sasakuma Tetsuo
Graduate School Of Integrated Science Yokohama City University
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Sasanuma Tsuneo
Graduate School Of Integrated Science & Kihara Institute For Biological Research Yokohama City U
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Lang Yaqin
Graduate School of Integrated Science & Kihara Institute for Biological Research, Yokohama City Univ
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Yanagawa Satoshi
Tokita Seed Co.
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Lang Ya-qin
Graduate School Of Integrated Science & Kihara Institute For Biological Research Yokohama City U
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Sasakuma Tetsuo
Graduate School of Integrated Science & Kihara Institute for Biological Research, Yokohama City University
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