Orange Fruit Color in Capsicum due to Deletion of Capsanthin-capsorubin Synthesis Gene
スポンサーリンク
概要
- 論文の詳細を見る
Fruit color of pungent pepper (Capsicum annuum L.) is a trait of economic importance in pepper breeding. To develop a system of molecular marker-assisted selection (MAS) for breeding pepper varieties with various fruit colors, we conducted molecular genetic analyses on six genes involved in the carotenoid biosynthesis pathway. Capsanthin-capsorubin synthase (CCS) gene showed a polymorphism in the PCR pattern in the segregation population derived from a cross between a pepper accession (cv. msGTY-1) with orange fruits and a pepper accession (cv. 277long) with red fruits. A deletion was found in the upstream region of the CCS gene in the plants with orange fruits. Southern hybridization analysis and sequencing analysis indicated that 211-bp of the downstream region of the gene was conserved in the plants with orange fruits, while no transcript of the CCS gene was detected by RT-PCR in the mature orange fruits. Carotenoid composition analysis using the Thin layer chromatography (TLC) method showed that one of the major pepper carotenoids, capsanthin, was present in the red fruits, but not in the orange fruits. The PCR polymorphism of the CCS gene and TLC pattern of carotenoid composition were completely cosegregated with the fruit color in the F_2 population, suggesting that the CCS gene determines the fruit color by changing the carotenoid composition.
- 日本育種学会の論文
- 2004-03-01
著者
-
Sasakuma Tetsuo
Kihara Institute For Biological Research And Graduate School Of Integrated Science Yokohama City Uni
-
Sasakuma Tetsuo
Graduate School Of Integrated Science Yokohama City University
-
Sasanuma Tsuneo
Graduate School Of Integrated Science & Kihara Institute For Biological Research Yokohama City U
-
Yanagawa Satoshi
Tokita Seed Co.
-
Lang Ya-Qin
Graduate School of Integrated Science & Kihara Institute for Biological Research, Yokohama City Univ
-
Lang Ya-qin
Graduate School Of Integrated Science & Kihara Institute For Biological Research Yokohama City U
-
Sasakuma Tetsuo
Graduate School of Integrated Science & Kihara Institute for Biological Research, Yokohama City University
関連論文
- O-02. Construction of a transformation-competent artificial chromosome (TAC) library for lettuce molecular cytogenetics research(Abstracts of the oral and poster presentations)(The 53rd Annual Meeting of the Society of Chromosome Research)
- QTL analysis of fertility-restoration against cytoplasmic male sterility in wheat
- QTLs Associated with Plant Height and Related Characters in Hexaploid Wheat
- Identification of individual barley chromosomes based on repetitive sequences : Conservative distribution of Afa-family repetitive sequences on the chromosomes of barley and wheat
- A Gene Encoding a Putative Acyl-transferase Involved in Pungency of Capsicum
- Orange Fruit Color in Capsicum due to Deletion of Capsanthin-capsorubin Synthesis Gene
- Development of genomic in situ hybridization method to Technovit 7100 sections of wheat early embryo
- Repetitive DNA sequences locating on the polymorphic C-band region found in a novel chromosome of rye, Secale cereale(Abstracts of the 48th Annual Meeting of the Society of Chromosome Research)
- Early Heading Mutants of T.monococcum and Ae.squarrosa, A-and D-Genome Ancestral Species of Hexaploid Wheat