ペプシンとパンクレアチンを用いた酵素分析法のキノコ食物繊維定量への応用

概要

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A modified pepsin/pancreatin method was applied for the determination of dietary fibers in cultivated type mushroom. The results of histochemical examinations indicate that the hypha, basidium and basidiospore cell walls of the sample mushroom contained chitin but no cellulose, lignin nor starch. A small amount of insoluble pectin was observed in the hymeiuym. These cell wall components showed no change after the pepsin/ pancreatin treatment. On the other hand, protein reactions of protoplasm of various cells completely disappeared after the pepsin/ pancreatin digestion, except a few sclerenchyma and basophilic hyphae. The obtained data by this pepsin/ pancreatin method were compared with those by AOAC method in an analytical test. The total dietary fiber (TDF) values obtained by the pepsin/ pancreatin method were constantly higher than those by AOAC method.
1989-12-20