水溶性アゾ色素を用いるフローインジェクション分析法による血清中銅の迅速定量

概要

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TAMSMB {(2-(2-thiazolylazo)-4-methyl-5-sulfo-methyl-aminobenzoic acid} was used in a spectrophotometric flow injection system for the determination of copper in serum (0.7ppm to 1.5ppm). For the determination of copper in serum, a 20 μl of deproteinized sample is injected into a carrier stream of acetate buffer solution (pH 5) containing 0.1 % Brij-35. The carrier stream meets a reagent stream of TAMSMB (10^<-5>M) solution at confluence point. After mixing coil (0.5 mm i.d.× 200 cm), the absorbance at 585 nm of the effluent was continuously measured by a spectrophotometer with a 8 μl flow-through cell. Sampling rate was 70 samples per hour. Calcium(II), Magnesium(II), and iron(III) did not react with under the proposed condition. The method was satisfactorily applied to the variety of serum samples.
社団法人日本分析化学会の論文
1983-12-05