コロイド滴定法による電気泳動用寒天の負電荷含有量の測定

概要

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The negatively charged values of agar-agar for electrophoresis were determined by colloid titration. Anionic residues such as sulfate and carboxylate in agar-agar are responsible for electroendosmosis which causes poor resolution of migration pattern. Therefore, the measurement of negatively charged value is important for characterization of agar-agar for electrophoresis. Commercially available agar-agar is dried at 70 ℃ for 3 h in a vacuum. A 25 mg of the sample is accurately weighed and dissolved in hot water. To this solution, 10 ml of N/1600 polydiallyl dimethyl ammonium chloride are added and the solution is titrated with N/1600 potassium polyvinyl sulfate at 50 ℃ in order to prevent the gellation of agar-agar. The endpoint is indicated by a color change of toluidine blue from blue to red. Electroendosmosis was measured by comparison of the mobilities of BSA and starch.
社団法人日本分析化学会の論文
1984-02-05