Monomethylarsonous Acid Inhibits Endothelial Nitric Oxide Synthase Activity
スポンサーリンク
概要
- 論文の詳細を見る
Inorganic arsenic undergoes extensive reduction and oxidative methylation in cells to yield a reactive metabolite of inorganic arsenic monomethylarsonous acid (MMA^<III>), which has a high reactivity toward vicinal thiols. Our epidemiological study in an endemic area of chronic arsenic poisoning and in experiments with rabbits exposed to arsenic revealed that arsenic exposure results in a reduction of systemic nitric oxide (NO) production. In this study, we examined the effect of MMA^<III> on endothelial NO synthase (eNOS) activity. With the membrane fraction of bovine aortic endothelial cells (BAEC), it was found that MMA^<III> with an IC_<50> value of 2.1μM was a potent inhibitor of eNOS, whereas inorganic arsenic and their methylated metabolites had no effect on eNOS activity. Interestingly, addition of dithiothreitol markedly blocked the MMA^<III>-induced inhibition of eNOS activity. This report is the first to suggest that MMA^<III> interacts with eNOS protein through presumably vicinal thiols, leading to decreased eNOS activity.
- 公益社団法人日本薬学会の論文
- 2005-12-01
著者
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TAGUCHI Keiko
Department of Geriatric Medicine, Ehime University Graduate School of Medicine
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SUN Yang
Department of Chemical Engineering, Hanyang University
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Sumi Daigo
Department of Geriatrics, Nagoya University School of Medicine
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Sumi Daigo
Faculty Of Pharmaceutical Sciences Tokushima Bunri University
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Sumi Daigo
Department Of Geriatrics Nagoya University School Of Medicine
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Sumi Daigo
Master's Program In Environmental Sciences University Of Tsukuba:doctoral Programs In Medical S
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KUMAGAI Yoshito
Department of Environmental Medicine, Institute of Community Medicine, University of Tsukuba
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Shinkai Yasuhiro
Doctoral Program In Life System Medical Sciences Graduate School Of Comprehensive Human Sciences Uni
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Shinkai Yasuhiro
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Kumagai Yoshito
Doctoral Program In Life System Medical Sciences Graduate School Of Comprehensive Human Sciences Uni
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Kumagai Yoshito
Master's Program In Environmental Sciences University Of Tsukuba:doctoral Programs In Medical S
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Kumagai Yoshito
Department Of Environmental Medicine Institute Of Community Medicine
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Shinkai Yasuhiro
Department of Environmental Medicine, Doctoral Programs in Medical Sciences, Graduate School of Comp
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Taguchi Keiko
Department Of Environmental Medicine Doctoral Programs In Medical Sciences Graduate School Of Compre
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Sun Yang
Department Of Chemical Engineering Hanyang University
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Sun Yang
Department Of Environmental Medicine Doctoral Programs In Medical Sciences Graduate School Of Compre
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