1,2-Naphthoquinone suppresses lipopolysaccharide-dependent activation of IKKβ/NF-κB/NO signaling: an alternative mechanism for the disturbance of inducible NO synthase-catalyzed NO formation

スポンサーリンク

概要

• 論文の詳細を見る

• 1,2-Naphthoquinone (1,2-NQ) is an uncoupling agent for constitutive nitric oxide (NO) synthase (NOS), thereby inhibiting its catalytic activity. However, little information on whether this quinone can affect inducible NOS (iNOS) is available. To address this issue, we examined the effect of 1,2-NQ on lipopolysaccharide (LPS)-mediated induction of iNOS. Exposure of LPS-challenged RAW264.7 cells to 1,2-NQ resulted in decreased NO formation through a reduction in iNOS production. Under these conditions, LPS-induced activation of nuclear transcription factor-κB (NF-κB) coupled to phosphorylation of inhibitory κBα (IκBα) declined. Similar effects of 1,2-NQ were observed in the lungs of mice exposed to LPS. Using IκB kinase β (IKKβ)-transfected RAW264.7 cells and recombinant IKKβ protein, we found that 1,2-NQ diminished the phosphorylation of IκB by IKKβ enzymatic activity. Taken together, these results suggest that 1,2-NQ reduces iNOS-catalyzed NO production through 1) an uncoupling reaction, as reported previously, and/or 2) disruption of IKKβ/NF-κB signaling.

• 日本トキシコロジー学会の論文

著者

• Sumi Daigo

  Master's Program In Environmental Sciences University Of Tsukuba:doctoral Programs In Medical S

• Akimori Masako

  Master’s Program in Environmental Sciences, University of Tsukuba

• Inoue Ken-ichiro

  Environmental Health Sciences Division, National Institute for Environmental Studies

• Takano Hirohisa

  Environmental Health Sciences Division, National Institute for Environmental Studies

• Kumagai Yoshito

  Master’s Program in Environmental Sciences, University of Tsukuba

関連論文

• Catechol estrogens mediated activation of Nrf2 through covalent modification of its quinone metabolite to Keap1
• Reduction of arsenic-induced cytotoxicity through Nrf2/HO-1 signaling in HepG2 cells
• 1,2-Naphthoquinone suppresses lipopolysaccharide-dependent activation of IKKβ/NF-κB/NO signaling: an alternative mechanism for the disturbance of inducible NO synthase-catalyzed NO formation
• 1,2-Naphthoquinone suppresses lipopolysaccharide-dependent activation of IKKβ/NF-κB/NO signaling : an alternative mechanism for the disturbance of inducible NO synthase-catalyzed NO formation
• Arsenic and Other Metal Contamination of Groundwater in the Mekong River Delta, Vietnam
• Monomethylarsonous Acid Inhibits Endothelial Nitric Oxide Synthase Activity
• Peroxiredoxin 6 is a molecular target for 1,2-naphthoquinone, an atmospheric electrophile, in human pulmonary epithelial A549 cell
• Peroxiredoxin 6 is a molecular target for 1,2-naphthoquinone, an atmospheric electrophile, in human pulmonary epithelial A549 cells

スポンサーリンク