Identification of four species of the Anopheles hyrcanus complex (Diptera : Culicidae) found in Korea using species-specific primers for Polymerase Chain Reaction Assay
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概要
- 論文の詳細を見る
For identification of four sibling species of the Anopheles hyrcanus complex found in Korea, the 5.8 rDNA-ITS2-28S rDNA region of each species was sequenced and the species-specific primers were designed. The amplified PCR products obtained from each species were analyzed by electrophoresis on 3% agarose gel. The result showed a single species-specific band, i.e. 559bp, 432bp, 322bp and 192bp for An. sinensis, An. sp., An. lesteri and An. pullus, respectively. In conclusion, the species-specific PCR primers designed from ITS2 variable regions functioned successfully and specifically, and can be applied as a useful tool for identifying species of the Hyrcanus complex found in Korea.
- 日本衛生動物学会の論文
- 2005-09-15
著者
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Ree Han
Institute Of Tropical Medicine & Department Of Parasitology College Of Medicine Yonsei Universit
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Hwang Ui
Institute Of Tropical Medicine & Department Of Parasitology College Of Medicine Yonsei Universit
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YONG Tai
Institute of Tropical Medicine & Department of Parasitology, College of Medicine, Yonsei University
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Yong Tai
Institute Of Tropical Medicine & Department Of Parasitology College Of Medicine Yonsei Universit
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Hwang Ui-Wook
Institute of Tropical Medicine & Department of Parasitology, College of Medicine, Yonsei University:Department of Biology, Teachers College, Kyungpook National University
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Ree Han-Il
Institute of Tropical Medicine & Department of Parasitology, College of Medicine, Yonsei University
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Yong Tai-Soon
Institute of Tropical Medicine & Department of Parasitology, College of Medicine, Yonsei University
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- Identification of four species of the Anopheles hyrcanus complex (Diptera : Culicidae) found in Korea using species-specific primers for Polymerase Chain Reaction Assay