Cell Placement and Neural Guidance Using a Three-Dimensional Microfluidic Array : Instrumentation, Measurement, and Fabrication Technology

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概要

• 論文の詳細を見る

• Several fabrication techniques for making three-dimensional arrays of micro-wells for biological cell patterning and single-neuron guidance are presented. Methods for making complex 3d high-aspect-ratio structures in poly-dimethylsiloxane (PDMS) membrane are explored. In this work, three-dimensional micro-molds are made directly on silicon wafers though in-ductively coupled plasma reactive ion etching (ICP-RIE), and also using SU-8 negative photoresist. Cell placement is achieved through an array of 50-μm square holes in a 150-100 μm thick PDMS membrane, which is placed on a glass substrate. Vertical holes in the membrane are linked by horizontal tunnels on the glass side of the membrane, for use in neural guidance or delivery of drugs or nutrients. The effectiveness of the membrane for cell placement, growth and guidance was tested using fluorescent yeast cells and PC12 neuronal cells.

• 社団法人応用物理学会の論文
• 2001-09-15

著者

• DEGENAAR Patrick

  Japan Advanced Institute of Science and Technology School of Materials Science

• Fujita Hiroyuki

  Limms Cnrs-iis University Of Tokyo

• GRISCOM Laurent

  LIMMS CNRS-IIS, University of Tokyo

• LEPIOUFLE Bruno

  BIOMIS, ENS de Cachan Antenne de Bretagne, Campus de Ker-lann

• TAMIYA Eichi

  Japan Advanced Institute For Science And Technology

• Griscom Laurent

  Limms Cnrs-iis University Of Tokyo

• Lepioufle Bruno

  Biomis Ens De Cachan Antenne De Bretagne Campus De Ker-lann

• Degenaar P

  Japan Advanced Inst. Of Sci. And Technol. School Of Materials Sci. Ishikawa

• Degenaar Patrick

  Japan Advanced Institute For Science And Technology

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