Sensitive Liquid Chromatographic Assay for the Simultaneous Determination of Ibuprofen and Its Prodrug, Ibuprofen Eugenol Ester, in Rat Plasma
スポンサーリンク
概要
- 論文の詳細を見る
A rapid, sensitive, and specific reverse-phase high-performance liquid chromatography (HPLC) method was developed to quantitate ibuprofen (IBU) and its prodrug, ibuprofen eugenol ester (IEE), simultaneously in rat plasma. IBU, IEE, and the internal standard glycryrrhetic acid (GA) were detected by UV absorption at 230nm. Extraction recoveries for all compounds ranged between 82.6% and 96.2%. Retention times of IBU, IEE, and GA were 5.62, 15.98, and 18.05min, respectively. Calibration plots were linear over the range of 0.64 to 64μg/ml for IBU, and 0.16 to 80μg/ml for IEE. The limit of quantitation was 0.64μg/ml for IBU and 0.16μg/ml for IEE. The intra- and interday variations were less than 10% and accuracy was greater than 90%. The results showed that the established method is reproducible and sensitive and applicable to plasma samples collected from rats administered IBU and IEE intravenously.
- 公益社団法人日本薬学会の論文
- 2005-09-01
著者
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Chen Dawei
School Of Pharmacy Shenyang Pharmaceutical Univ.
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Chen Dawei
Department Of Pharmaceutics School Of Pharmaceutical Science Shenyang Pharmaceutical University
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ZHAO XiuLi
Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University
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Zhao Xiuli
School Of Pharmacy Shenyang Pharmaceutical Univ.
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LI Kexin
School of Pharmacy, Shenyang Pharmaceutical University
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WANG Danlei
School of Pharmacy 42 mailbox, Shenyang Pharmaceutical University
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Wang Danlei
School Of Pharmacy 42 Mailbox Shenyang Pharmaceutical University
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