High-Cell-Density Fermentation for Ergosterol Production by Saccharomyces cerevisiae(BIOCHEMICAL ENGINEERING)

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概要

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• The direct feedback control of glucose using an on-line ethanol concentration monitor for ergosterol production by high-cell-density fermentation was investigated and the fermentation parameters (e.g., pH, dissolved oxygen, ethanol concentration, oxygen uptake rate, carbon dioxide evolution rate and respiratory quotient) were analyzed. Controlling glucose feeding rate in accordance with ethanol concentration and adjusting pH with ammonia during the fermentation process were effective fed-batch methods for ergosterol production. The fermentation parameters well described the variation of the whole fermentation process. Cultivation in a 5l fermentor was carried out under the following conditions : culture temperature, 30℃; pH, 5.5; agitation speed, 600rpm; fermentation time, 60h; controlling ethanol concentration below 1% and keeping respiratory quotient (RQ) at approximately 1.0. Under these conditions, the yeast dry weight reached 120g/l and the ergosterol yield reached 1500mg/l.

• 2006-01-25

著者

• Wang Xi

  Beijing Key Lab Of Bioprocess College Of Biology Science And Technology Beijing University Of Chemic

• Shang Fei

  Beijing Key Lab of Bioprocess, College of Biology Science and Technology, Beijing University of Chem

• Wen Shaohong

  Ocean School, Yantai University

• Tan Tianwei

  Beijing Key Lab of Bioprocess, College of Biology Science and Technology, Beijing University of Chem

• Shang Fei

  Beijing Key Lab Of Bioprocess College Of Biology Science And Technology Beijing University Of Chemic

• Tan Tianwei

  Beijing Key Lab Of Bioprocess College Of Biology Science And Technology Beijing University Of Chemic

• Tan Tianwei

  Beijing Key Lab Of Biochemical Engineering Beijing University Of Chemical Technology

• Wen Shaohong

  Ocean School Yantai University

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