Stimulatory effect of interleukin-6 on both cathepsins B and L activities from human periodontal ligament cells

スポンサーリンク

概要

• 論文の詳細を見る

• Cathepsin is typical and well-characterized lysosomal cystein protease and in pathological conditions is involved in tissue destruction. Recent studies have reported that the mRNA expression of cathepsin K, a family of cystein proteases such as cathepsin B (CAB) and cathepsin L (CAL), is detected in the osteoclasts on the pressure side of the alveolar bone during experimental tooth movement. Interleukin (IL)-6 is a multifunctional cytokine that induces osteoclastic bone resorption through an effect on osteoclastgenesis. PDL (periodontal ligament) cells have produced some important factors such as IL-6 in response to IL-1 β in the periodontal metabolism. There are few studies on the role of cathepsin in PDL cells. In this study, we examined the effect of IL-6 on both CAB and CAL activities of human PDL cells. IL-6 treatment induced significantly higher activities of CAB and CAL in PDL cells in a time-and dose-dependent manner, compared with non-treated controls. The findings of the RT-PCR analysis showed that the levels of RT-PCR products for CAB and CAL obtained from the IL-6-treated cells were higher than those obtained from control cells. These results suggested that the increased activities of CAB and CAL by IL-6 seemed to be the respective induction of the expression of CAB and CAL mRNA, which may be involved in extracellular matrix degradation in the periodontal metabolism.

• 日本矯正歯科学会の論文

著者

• Kazutaka Kasai

  Departments Of Orthodontics Nihon University School Of Dentistry At Matsudo

• Masaru Yamaguchi

  Department Of Orthodontics Nihon University School Of Dentistry At Matsudo

• Yuji Ueda

  Departments Of Orthodontics Nihon University School Of Dentistry At Matsudo

関連論文

• Localization of RANKL and cathepsin K, B and L in rat periodontal tissues during experimental tooth movement
• Cathepsins B and L Increased during Response of Periodontal Ligament Cells to Mechanical Stress In Vitro
• The level of cathepsin L in gingival crevicular fluid during orthodontic tooth movement and effect of tension-force on cathepsin L activity from periodontal ligament cells
• Relaxin Modulates Collagen Type I and Matrix Metalloproteinase-1 Expression by Human Periodontal Ligament Cells
• Stimulatory effect of interleukin-6 on both cathepsins B and L activities from human periodontal ligament cells

スポンサーリンク