モルモットの歯肉固有層におけるオキシタラン線維の組織学的観察

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The gingiva is mucosal tissue composed of gingival epithelium and gingival lamina propria. The present study examined the distribution and ratio of oxytalan fibers of gingival lamina propria related to aging and types of the diet in guinea pig. A group of 8 guinea pigs was weaned at 8 weeks of age (Young group) and another group of 8 guinea pigs was weaned at 60 weeks of age (Old group) . Each group was divided into two groups ; they were fed 8 weeks with either soft diet or hard diet. The frontal sections of the mandibular incisor region were prepared and stained with H.E. stain, Masson's trichrome stain and the Weigert's resorcin fuchsin preoxidation method ; preoxidation was performed in a solution containing O . 3% H_2S0_4 and O . 3% KMn0_4 for 30 min. Oxytalan fibers in the gingival lamina propria were determined quantitatively with the computer-assisted image analyzer system. The results showed pathological changes of collagen fibers in old animals exposed to the soft diet. Concerning the ratio of oxytalan fibers, a prominent increment was particularly observed in the old groups. On the other hand, no significant differences were found in young groups fed with different diets. This study indicated a tissue repair mechanism by compensation of oxytalan fibers for collagen fiber loss in gingival lamina propria of old group fed with soft diet. J. Jpn. Soc. Periodontol., 41 : 118-124, 1999.
特定非営利活動法人日本歯周病学会の論文
1999-06-28

モルモットの歯肉固有層におけるオキシタラン線維の組織学的観察

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