歯周病細菌によるヒト末梢血T細胞活性化

概要

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To examine the host-parasite interactions of naive helper T cells and periodontal bacteria in the onset of periodontal disease, the mode of activation of T cells from periodontally healthy donors in response to Porphyromonas gingivalis (Pg) was studied. Peripheral blood mononuclear cells from 10 donors were stimulated by co-culture with Pg whole cells or Pg lipopolysaccharide (LPS) for 7-10 days. Activated T cells were recognized as enlarged cells or cells with increased intracellular DNA. The number of activated T cells after the culture period differed with the donor which confers association with HLA class II genotype. The activation of T cells in response to Pg whole cells was completely inhibited by the addition of anti HLA-DR antibody in stimulation culture, strongly suggesting that the activation was specific to HLA-DR molecules. The percentage of activated T cells in response to PgLPS was 10% at most of that in response to Pg whole cells. The number of CD45RO^+CD4^+ cells was markedly increased and the number of CD45RA^+CD4^+ cells decreased in activated T cells in response to Pg whole cells or positive stimulation. These findings suggest that naive helper T cells which migrate from peripheral blood to inflamed periodontal tissue recognize Pg antigen in HLA-DR specific manner, proliferate, and undergo phenotype transformation from CD45RA to CD45RO.
福岡歯科大学学会の論文
1997-09-30